RAPID COMMUNICATION Induction of Human Monocyte to Macrophage Maturation In Vitro
نویسنده
چکیده
Cells of the mononuclear phagocyte system arise from circulating blood monocytes (MO) that undergo further maturation on leaving the vasculature and migration into the various tissues and body cavities. This terminal differentiation step is also observed in vitro when blood MO are cultured in the presence of serum. Yet, the inducing signals present in serum are not defined. We have established primary cultures from elutriation-purified blood MO and found that the active metabolite of vitamin D, 1.25dihydroxyvitamin D, (1,25(OH),D,) could induce maturation of MO to macrophages (MAC) in the absence of any serum proteins. Cells were cultured for 7 days with AB-group serum or ,1 ,25(OH),D,, respectively, and MO maturation
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